MUC2 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1151989
• Category: Antibodies
• Description: MUC2 Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 7F9
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MUC2
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1:50-100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 570 kDa
• Target: MUC2
• UniProt ID: Q02817
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: MLP; SMUC; Intestinal mucin 2; MUC 2; Mucin 2; Muc
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Zhaoxi Deng a b , Yanfei Ma b , Kangwei Hou b , Jinyan Dai b , Yifan Zhong b , Yingying Mao b , Jinzhi Zhang b , Haifeng Wang Comparison of Lactobacillus rhamnosus GG and luxS mutant on gut barrier function of mice challenged with Escherichia coli Journal of Functional Foods, (2025)

PubMed:

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: Rat colon, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 540 kDa, Observed MW: 140-170, > 250 kDa.

Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 90% methanol, Primary ab Dilution: 1:100, Secondary ab: Goat anti Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb1151989.

Cell line: NIH/3T3, Fixative: 4% Paraformaldehyde, Permeabilization: 90% methanol, Primary ab Dilution: 1:100, Secondary ab: Goat anti Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb1151989.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (MUC2) Monoclonal Antibody, Unconjugated (orb1151989) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (MUC2) Monoclonal Antibody, Unconjugated (orb1151989) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human duodenum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (MUC2) Monoclonal Antibody, Unconjugated (orb1151989) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.