MUC2 Antibody / Mucin 2

• Catalog Number: orb639822
• Category: Antibodies
• Description: Recognizes a single glycoprotein of 520kDa, identified as mucin 2 (MUC2). This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium. MUC2 is specifically expressed in goblet cells of the small intestine in about 65% of colonic carcinomas and about 40% of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: Amino acids KYPTTTPISTTTMVTPTPTPTGTQTPTTT were used as the immunogen for this recombinant MUC2 antibody. Its epitope has been defined as GTQTP.
• UniProt ID: Q02817
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: rMLP/842
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Store the recombinant MUC2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Hazard Information: This recombinant MUC2 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human stomach with recombinant MUC2 antibody (clone rMLP/842). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human stomach with recombinant MUC2 antibody (clone rMLP/842). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant MUC2 antibody (clone rMLP/842) as confirmation of integrity and purity.