MUC1 / EMA antibody

SKU: orb2276500

Description

MUC1 / EMA antibody validated for immunohistochemistry on 76 different Normal Tissues

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:75-1:150
• Reactivity: Human
• Application Notes:
  Positive Control: Kidney: Distal tubuli and collecting ducts must show a strong cytoplasmic MUC1 staining while the staining should be at least moderate in the parietal and visceral sheets of the Bowman’s capsule and largely absent in proximal tubuli. Negative Control: Colon: MUC1 staining should be very weak or absent in colonic epithelium. Cellular Localization: Cytoplasmic & Cell Surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit according to the manufacturer’s directions.

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Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: MSVA-672R
• Immunogen: Recombinant human MUC1 protein
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma- associated mucin Episialin, Epithelial Membrane Antigen (EMA), H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1- alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface associated, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen

Quick Database Links

UniProt

P15941