MUC1 Antibody / Mucin-1

• Catalog Number: orb533984
• Category: Antibodies
• Description: Mucin-1/MUC1 protein is encoded by the MUC1 gene in humans. This gene encodes a membrane-bound protein that is a member of the mucin family. Family members are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. MUC1 is a transmembrane protein normally expressed on the apical borders of secretory epithelial cells. Overexpression is often associated with colon, breast, ovarian, lung and pancreatic cancers. The dominant epitope of this MAb involves both amino acids as well as sugar moieties. Its epitope is destroyed by desialylation i.e. treatment with Neuraminidase.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: MCN01-2R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Human milk fat globule membranes were used as the immunogen for this recombinant MUC1 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant MUC1 antibody is available for research use only.
• Entrez: 4582
• UniProt ID: P15941
• Storage: Store the recombinant MUC1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titration of the recombinant MUC1 may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.