MTX1 Antibody

SKU: orb1256098

Description

MTX1 Antibody

Research Area

Cancer Biology, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 186-435 of human MTX1 (NP_942584.2).
• Target: MTX1
• Molecular Weight: Observed: 35kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MTX, MTXN, metaxin-1, mitochondrial outer membrane import complex protein 1

Western blot analysis of extracts of various cell lines, using MTX1 antibody (orb1256098) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human colon using MTX1 antibody (orb1256098) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using MTX1 antibody (orb1256098) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse stomach using MTX1 antibody (orb1256098) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using MTX1 antibody (orb1256098) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

MTX1

UniProt

Q13505