mtTFA rabbit pAb

• Catalog Number: orb770250
• Category: Antibodies
• Description: Rabbit polyclonal antibody to mtTFA
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Bovine, Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human TFAM. AA range:131-180
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: WB 1:500-2000;Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-TFAM antibody, anti-TCF6 antibody, anti-TCF6L
• Note: For research use only

Immunofluorescence analysis of HepG2 cells, using TFAM Antibody. The picture on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of Hela cell. 1, mtTFA Polyclonal Antibody (Green) was diluted at 1:200 (4°C overnight). Histone H3 (Tri Methyl Lys36) Monoclonal Antibody (Q12) (Red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Western blot analysis of 293T 3T3 lysis using mtTFA antibody. Antibody was diluted at 1:500.

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using TFAM Antibody. The picture on the right is blocked with the synthesized peptide.