mtTFA rabbit pAb Antibody

SKU: orb770250

Description

Rabbit polyclonal antibody to mtTFA

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: WB 1:500-2000;Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Bovine, Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human TFAM. AA range:131-180
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-TFAM antibody, anti-TCF6 antibody, anti-TCF6L2 antibody, anti-Transcription factor A antibody, anti- mitochondrial antibody, anti-mtTFA antibody, anti-Mitochondrial transcription factor 1 antibody, anti-MtTF1 antibody, anti-Transcription factor 6 antibody, anti-TCF-6 antibody, anti-Transcription factor 6-like 2 antibody

Immunofluorescence analysis of HepG2 cells, using TFAM Antibody. The picture on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of Hela cell. 1, mtTFA Polyclonal Antibody (Green) was diluted at 1:200 (4°C overnight). Histone H3 (Tri Methyl Lys36) Monoclonal Antibody (Q12) (Red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Western blot analysis of 293T 3T3 lysis using mtTFA antibody. Antibody was diluted at 1:500.

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using TFAM Antibody. The picture on the right is blocked with the synthesized peptide.