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| Catalog Number | orb764311 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to mTOR (phospho-Thr2446) |
| Clonality | Polyclonal |
| Tested applications | ELISA, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human mTOR around the phosphorylation site of Thr2446. AA range:2412-2461 |
| Concentration | 1 mg/ml |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Conjugation | Unconjugated |
| MW | 220 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-MTOR antibody, anti-FRAP antibody, anti-FRAP1 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western Blot analysis of various cells using Phospho-mTOR (T2446) Polyclonal Antibody diluted at 1:1000.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using mTOR (Phospho-Thr2446) Antibody.
Western blot analysis of lysates from NIH/3T3 cells treated with Insulin 0.01U/ml 15', using mTOR (Phospho-Thr2446) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Phospho-mTOR (Thr2446) Rabbit pAb [orb2715587]
WB
Human, Mouse, Rat
Polyclonal
Unconjugated
20 μl, 100 μl, 50 μl
