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Catalog Number | orb768271 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to mTOR (phospho-Ser2481) |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P |
Reactivity | Bovine, Human, Mouse, Rat |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human mTOR around the phosphorylation site of Ser2481. AA range:2447-2496 |
Concentration | 1 mg/ml |
Dilution range | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. ,WB 1:500-2000 |
Conjugation | Unconjugated |
MW | 289kD |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-MTOR antibody, anti-FRAP antibody, anti-FRAP1 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot analysis of hela-UV using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using mTOR (Phospho-Ser2481) Antibody.
Immunofluorescence analysis of NIH/3T3 cells, using mTOR (Phospho-Ser2481) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using mTOR (Phospho-Ser2481) Antibody. The picture on the right is blocked with the phospho peptide.
FC, ICC, IF | |
Canine, Equine, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
FITC |
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF | |
Canine, Equine, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Pacific Blue |
FC, ICC, IF | |
Canine, Equine, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
BF700 |
WB | |
Canine, Equine, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
IRDye800 |