mTOR (FRAP1) Antibody (S2481)

SKU: orb1930132

Description

mTOR (FRAP1) Antibody (S2481)

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Immunology & Inflammation, Metabolism Research

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: WB - 1:2000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 288892 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Confocal immunofluorescent analysis of mTOR (FRAP1) Antibody (S2481) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human testis tissue reacted with FRAP1-pS2481, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of FRAP1 (arrow) using rabbit polyclonal FRAP1 Antibody (S2481). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FRAP1 gene (Lane 2).

All lanes: Anti-FRAP1 Antibody (pS2481) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 289 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P42345