mTOR Antibody

• Catalog Number: orb1258164
• Category: Antibodies
• Description: mTOR Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human mTOR (NP_004949.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 289kDa
• Target: mTOR
• UniProt ID: P42345
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: TOR Antibody: FRAP, FRAP1, FRAP2, RAFT1, RAPT1, FR
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using mTOR antibody (orb1258164) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunofluorescence analysis of HeLa cells using mTOR antibody (orb1258164) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC12 cells using mTOR antibody (orb1258164) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using MTOR antibody (orb1258164) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using MTOR antibody (orb1258164) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug mTOR antibody (orb1258164). Western blot was performed from the immunoprecipitate using mTOR antibody (orb1258164) at a dilution of 1:1000.