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Catalog Number | orb1249270 |
---|---|
Category | Antibodies |
Description | MTHFD1L Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Predicted Reactivity | Bovine, Canine, Porcine |
Reactivity | Human, Mouse, Rat |
Immunogen | The immunogen for this antibody is: C-DTNDRFLRKITIGQ |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 110kDa band observed in Human Brain (Cerebellum) (calculated MW of 106kDa according to NP_056255.2). The same band is observed in lysates of cell line NIH3T3, Mouse Brain and Rat Brain. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig for this particular batch.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | MTHFD1L |
UniProt ID | Q6UB35 |
NCBI | NP_056255.2 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | MTHFD1L, methylenetetrahydrofolate dehydrogenase ( Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 110kDa band observed in Human Brain (Cerebellum) (calculated MW of 106kDa according to NP_056255.2). The same band is observed in lysates of cell line NIH3T3, Mouse Brain and Rat Brain. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig for this particular batch.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. |
Expiration Date | 12 months from date of receipt. |
orb1249270 (0.1 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1249270 (0.1 ug/ml) staining of NIH3T3 (A), Mouse Brain (B) and Rat Brain (C) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1249270 (5 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ELISA, IHC, WB | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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