MSX1 Antibody (Center)

• Catalog Number: orb1932464
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB30384
• Tested applications: IF, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, IF: 1:200, WB: 1:1000, WB: 1:2000, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 31496 Da
• Target: This MSX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-138 amino acids from the Central region of human MSX1.
• UniProt ID: P28360
• NCBI: NP_002439.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Homeobox protein MSX-1, Homeobox protein Hox-7, Ms
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Fluorescent confocal image of SY5Y cells stained with MSX1 (Center) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MSX1 (Center) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).

Fluorescent image of U251 cell stained with MSX1 Antibody (Center).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MSX1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C).MSX1 immunoreactivity is localized to Nucleus significantly.

MSX1 Antibody (Center) western blot analysis in A375 cell line lysates (35 ug/lane). This demonstrates the MSX1 antibody detected the MSX1 protein (arrow).

MSX1 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35 ug/lane). This demonstrates the MSX1 antibody detected the MSX1 protein (arrow).

Anti-MSX1 Antibody (Center) at 1:2000 dilution + mouse spleen lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 31 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.