MSK1 (phospho Thr581) rabbit pAb

• Catalog Number: orb770873
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MSK1 (phospho-Thr581)
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human MSK1 around the phosphorylation site of Thr581. AA range:551-600
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 90
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-RPS6KA5 antibody, anti-MSK1 antibody, anti-Ri
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MSK1 (Phospho-Thr581) Antibody.

Immunofluorescence analysis of A549 cells, using MSK1 (Phospho-Thr581) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from RAW264.7 cells treated with UV 5', using MSK1 (Phospho-Thr581) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).