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MSK1 (phospho Ser212) rabbit pAb
Description
Images & Validation
−| Tested Applications | ELISA, IHC-P, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Reactivity | Human, Mouse |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MSK1 around the phosphorylation site of Ser212. AA range:181-230 |
| Molecular Weight | 90 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
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100 μlphospho-MSK1 (Ser212) Rabbit pAb, IRDye 800CW conjugated [orb2821741]
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100 μlPhospho-MSK1 (Ser212) Rabbit pAb [orb2711187]
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Western Blot analysis of 3T3 cells using Phospho-MSK1 (S212) Polyclonal Antibody diluted at 1:1000.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MSK1 (Phospho-Ser212) Antibody.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MSK1 (Phospho-Ser212) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 5', using MSK1 (Phospho-Ser212) Antibody. The lane on the right is blocked with the phospho peptide.
Quick Database Links
Protocol Information
MSK1 (phospho Ser212) rabbit pAb (orb770874)
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