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Catalog Number | orb1258096 |
---|---|
Category | Antibodies |
Description | MSI1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Rat |
Isotype | IgG |
Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MSI1 (NP_002433.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:50 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 47kDa |
Target | MSI1 |
UniProt ID | O43347 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | MSI1, Musashi homolog 1 (Drosophila), Musashi RNA- Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of A-549 cells, using MSI1 antibody (orb1258096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat lung using MSI1 antibody (orb1258096) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using MSI1 antibody (orb1258096) at dilution of 1:100. Blue: DAPI for nuclear staining.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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