MSH2 Antibody (Center)

SKU: orb1927243

Description

Mouse Monoclonal Antibody (Mab)

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:2000, IHC - 1:1000, IHC-P-Leica - 1:1000
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: B3306EV3X5X4
• Immunogen: Purified His-tagged MSH2 protein was used to produced this monoclonal antibody.
• Target: MSH2
• Molecular Weight: 104743 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA mismatch repair protein Msh2, hMSH2, MutS protein homolog 2, MSH2

MSH2 Antibody (Center) western blot analysis in 293, Hela cell line lysates (35μg/lane).This demonstrates the MSH2 antibody detected the MSH2 protein (arrow).

Immunohistochemical analysis of paraffin-embedded Human tonsil section using Pink1. Diluted at 1:1000 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded human appendix tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Quick Database Links

Gene Symbol

MSH2

UniProt

P43246

RefSeq (Protein):NP_000242.1, NP_001245210.1