MRPL28 Antibody

• Catalog Number: orb1257261
• Category: Antibodies
• Description: MRPL28 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-256 of human MRPL28 (NP_006419.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 30kDa
• Target: MRPL28
• UniProt ID: Q13084
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: 39S ribosomal protein L28, mitochondrial, L28mt, M
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry of paraffin-embedded rat ovary using MRPL28 antibody (orb1257261) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using MRPL28 antibody (orb1257261) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Immunohistochemistry of paraffin-embedded human lung cancer using MRPL28 antibody (orb1257261) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using MRPL28 antibody (orb1257261) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using MRPL28 antibody (orb1257261). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of 293T cells using 1 ug MRPL28 antibody (orb1257261). Western blot was performed from the immunoprecipitate using MRPL28 antibody (orb1257261) at a dilution of 1:1000.