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MRPL28 Antibody
SKU: orb1257261
Featured
Description
Images & Validation
−Item 1 of 6
| Tested Applications | IF, IHC, IP, WB |
|---|---|
| Reactivity | Human, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-256 of human MRPL28 (NP_006419.2). |
| Target | MRPL28 |
| Molecular Weight | Observed: 30kDa |
| Purification | Affinity purification |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−39S ribosomal protein L28, mitochondrial, L28mt, MRP-L28, Melanoma antigen p15, Melanoma-associated antigen recognized by T-lymphocytes, MRPL28, MAAT1
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Immunohistochemistry of paraffin-embedded rat ovary using MRPL28 antibody (orb1257261) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using MRPL28 antibody (orb1257261) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.
Immunohistochemistry of paraffin-embedded human lung cancer using MRPL28 antibody (orb1257261) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using MRPL28 antibody (orb1257261) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using MRPL28 antibody (orb1257261). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of 293T cells using 1 ug MRPL28 antibody (orb1257261). Western blot was performed from the immunoprecipitate using MRPL28 antibody (orb1257261) at a dilution of 1:1000.
Quick Database Links
Gene Symbol
MRPL28
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IP
Immunoprecipitation
MRPL28 Antibody (orb1257261)
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