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ABCC5 Antibody

SKU: orb1247251

Description

ABCC5 Antibody

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Porcine, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 150kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 161kDa according to NP_005679.2). Recommended concentration: 0.5-1.5µg/mL.Immunohistochemistry:In paraffin embedded Human Kidney shows membranous staining in DCT. Paraffin embedded Human Liver. Recommended concentration: 4-6µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: KDIDIGKEYIIP-C
TargetABCC5
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

MRP5, DKFZp686C1782, ABCC5, ATP-binding cassette, sub-family C (CFTR/MRP), member 5, ABC33, EST277145, MOAT-C, MOATC, SMRP, pABC11, ATP-binding cassette, sub-family C, member 5, canalicular multispecific organic anion transporter C

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ABCC5 Antibody

orb1247251 (0.5 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ABCC5 Antibody

orb1247251 (4 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Similar results with antigen retrieval at pH9.

ABCC5 Antibody

orb1247251 (5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Datasheet
Product Information
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

ABCC5 Antibody (orb1247251)

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0.1 mg
$ 920.00
DispatchUsually dispatched within 5-10 working days
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