MRP5 Antibody

• Catalog Number: orb1247251
• Category: Antibodies
• Description: MRP5 Antibody
• Target: ABCC5
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: KDIDIGKEYIIP-C
• UniProt ID: O15440
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 150kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 161kDa according to NP_005679.2). Recommended concentration: 0.5-1.5ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows membranous staining in DCT. Paraffin embedded Human Liver. Recommended concentration: 4-6ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MRP5, DKFZp686C1782, ABCC5, ATP-binding cassette,
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_005679.2, NP_001018881.1

orb1247251 (0.5 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247251 (4 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Similar results with antigen retrieval at pH9.

orb1247251 (5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.