MRE11 Antibody

SKU: orb345504

Description

Mre11 antibody

Images & Validation

• Tested Applications: ChIP, ELISA, WB
• Dilution Range: ELISA: 1:10,000 - 1:50,000, WB: 1:500- 1:2,000
• Reactivity: Yeast
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and by ChIP assay. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 575-600 of Saccharomyces cerevisiae (baker's yeast) Mre11 protein.
• Target: MRE11
• Purity: This affinity-purified antibody is directed against Saccharomyces cerevisiae Mre11 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that little cross reactivity should be expected with this protein from other sources.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.17 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Mre11 antibody, Double-strand break repair protein MRE11, DNA recombination and repair protein, Meiotic recombination 11

Chromatin Immunoprecipitation (ChIP) using Biorbyt's Affinity Purified Mre11 (S. cerevisiae) antibody. A yeast strain containing the HO endonuclease gene controlled by a galactose-inducible promoter (uninduced 0m lanes) was shifted into galactose containing medium (induced 60m lanes). After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of sheared chromatin. Chromatin was immunoprecipitated with the antibody at the stated dilutions. Immunocomplexes were captured using polyacrylamide bead linked secondary antibodies. The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower band) and 67 kb from the break (upper band, control locus).

Quick Database Links

Gene Symbol

MRE11

UniProt

P32829

RefSeq:6323880