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Catalog Number | orb1151514 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to MPO |
Clonality | Recombinant |
Species/Host | Mouse |
Isotype | Mouse IgG1, kappa |
Conjugation | Unconjugated |
Reactivity | Human |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Purity | Protein A/G affinity |
Immunogen | A portion of amino acids 150-250 was used as the immunogen for the recombinant MPO antibody. |
UniProt ID | P05164 |
Tested applications | IHC-P, WB |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 1-2ug/ml |
Application notes | Optimal dilution of the recombinant MPO antibody should be determined by the researcher. |
Antibody Type | Primary Antibody |
Clone Number | rMPO/6904 |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Storage | Aliquot the recombinant MPO antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Hazard Information | This recombinant MPO antibody is available for research use only. |
Note | For research use only |
Western blot testing of human spleen tissue lysate using recombinant MPO antibody (clone rMPO/6904). Expected molecular weight: 75-90 kDa (pro form).
SDS-PAGE analysis of purified, BSA-free MPO antibody (clone rMPO/6904) as confirmation of integrity and purity.
IHC staining of FFPE human bone marrow with recombinant MPO antibody (clone rMPO/6904). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant MPO antibody (clone rMPO/6904). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with recombinant MPO antibody (clone rMPO/6904). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.