Mouse Srms Antibody (Center)

• Catalog Number: orb1926643
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB50486
• Tested applications: IF, WB
• Reactivity: Mouse
• Isotype: Rabbit IgG
• Dilution range: IF: 1:25, WB: 1:1000, WB: 1:2000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 55758 Da
• Target: This Mouse Srms antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 113-147 amino acids from the Central region of Mouse Srms.
• UniProt ID: Q62270
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Tyrosine-protein kinase Srms, PTK70, Srms, Srm
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-Srms Antibody (C-term) at 1:2000 dilution + mouse liver lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 56 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of lysates from mouse liver, mouse spleen tissue lysate (from left to right), using Srms Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse skin tissue labeling Mouse Srms at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing cytoplasm staining on mouse skin tissue. The nuclear counter stain is DAPI (blue).