(Mouse) Sox17 Antibody (C-term)

• Catalog Number: orb1787992
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: 353-387 aa
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:25, WB: 1:2000, IHC: 1:25
• Conjugation: Unconjugated
• MW: 44646 Da
• Target: This mouse Sox17 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 353-387 amino acids from the C-terminal region of mouse Sox17.
• UniProt ID: Q61473
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Transcription factor SOX-17, Sox17, Sox-17
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-Sox17 Antibody (C-term) at 1:2000 dilution. Lane 1: F9 whole cell lysates. Lane 2: mouse testis lysates. Lane 3: LNCap whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on Hela cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red).

Staining (Mouse) Sox17 in mouse testis sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.