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Item 1 of 3
(Mouse) Pou5f1 Antibody (N-term)
Catalog Number: orb1926422
| Catalog Number | orb1926422 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB52378 |
| Tested applications | IHC-P, WB |
| Reactivity | Mouse |
| Isotype | Rabbit IgG |
| Dilution range | WB: 1:2000, WB: 1:2000, IHC-P: 1:25 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 38216 Da |
| Target | This Mouse Pou5f1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 86-119 amino acids from the N-terminal region of Mouse Pou5f1. |
| UniProt ID | P20263 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | POU domain, class 5, transcription factor 1, NF-A3 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Anti-Pou5f1 Antibody (N-term) at 1:2000 dilution + F9 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 38 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-Pou5f1 Antibody (N-term) at 1:2000 dilution + F9 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 38 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Staining (Mouse) Pou5f1 in mouse brain sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
