Mouse p27Kip1 Antibody (C-term T197)

SKU: orb1927538

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:1000
• Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This Mouse p27Kip1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-197 amino acids from the C-terminal region of mouse p27Kip1. Antigen Region: 175-197 aa.
• Target: Cdkn1b
• Molecular Weight: 22193 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, Cdkn1b

Mouse p27Kip1 Antibody (C-term T197) western blot analysis in mouse heart tissue lysates (35 ug/lane).This demonstrates the (mouse) p27Kip1 antibody detected the (mouse) p27Kip1 protein (arrow).

Anti-Mouse p27Kip1 Antibody (C-term T197) at 1:1000 dilution + C2C12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

Cdkn1b

UniProt

P46414

RefSeq (Protein):NP_034005.2