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Catalog Number | orb754972 |
---|---|
Category | Proteins |
Description | Recombinant mouse Noggin protein |
Tag | N-terminal His Tag or N-terminal His-IF2DI Tag, determined during production process |
Reactivity | Mouse |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4 |
Purity | Greater than 95% as determined by SDS-PAGE |
UniProt ID | P97466 |
MW | 22.4 kDa |
Tested applications | ELISA, WB |
Application notes | Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex |
Source | E.Coli |
Expression Region | 28-232 |
Storage | -20°C for 12 months as lyophilized;2-8°C for 1 month under sterile conditions after reconstitution |
Alternative names | Nog, NOGG_HUMAN, Noggin, SYM 1, SYM1, Symphalangis Read more... |
Note | For research use only |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
> 90% as determined by SDS-PAGE. | |
53 kDa |
> 95% as determined by SDS-PAGE | |
29-31 kDa |
> 90%, determined by SDS-PAGE | |
This protein contains the mouse NOG (P97466) (Met1-Cys232) was fused with the Fc region of human IgG1 at the C-terminus and expressed from HEK293 Cells. |
> 95% by SDS-PAGE. | |
Recombinant Mouse Noggin Protein with His Tag is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Gln28-Cys232) of mouse Noggin (Accession #NP_032737.1) fused with an 6��His Tag at the C-terminus. |