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Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Activity | Cell Based Assay: Mouse intestinal organoids were cultured with EGF (Cat#orb3002493), Noggin (Cat#orb3002448) and R-spondin 1 (Cat#orb3002497). After five days of culture, the organoids showed good morphology and germination rate. Mouse Gastric organoids were cultured with EGF (Cat#orb3002493), Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002448) , R-spondin 1 (Cat#orb3002497) and FGF-10 (Cat#orb3002478). The organoids showed good morphology. Mouse Colon organoids were cultured with EGF (Cat#orb3002493), Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002448) , R-spondin 1 (Cat#orb3002497) and FGF-10 (Cat#orb3002478). The organoids showed good morphology. |
| Tag | C-6His |
| Expression Region | Gln28-Cys232 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| MW | Predicted: 23.9 KDa. Observed: 30 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified). SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (Regularly tested) |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, 5mM EDTA, 5% Trehalose, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Noggin; Nog
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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