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Item 1 of 4
Mouse Nlrp6 Antibody (Center)
Catalog Number: orb1934705
| Catalog Number | orb1934705 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This Mouse Nlrp6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 343-372 amino acids from the Central region of mouse Nlrp6. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Mouse |
| Predicted Reactivity | Rat |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| UniProt ID | Q91WS2 |
| MW | 97402 Da |
| Tested applications | IHC-P, WB |
| Dilution range | WB: 1:500, WB: 1:2000, IHC-P-Leica: 1:500, IHC-P-Leica: 1:500 |
| Antibody Type | Primary Antibody |
| Clone Number | RB36346 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | NACHT, LRR and PYD domains-containing protein 6, A Read more... |
| Note | For research use only |
| NCBI | NP_598707.2 |
| Expiration Date | 12 months from date of receipt. |
Anti-NLRP6 Antibody at 1:2000 dilution + mouse heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 97 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-Mouse Nlrp6 Antibody (Center) at 1:500 dilution. Lane 1: Mouse small intestine lysate. Lane 2: Mouse liver lysate. Lane 3: Mouse spleen lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 97 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
