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Catalog Number | orb392186 |
---|---|
Category | Proteins |
Description | Recombinant mouse NGF protein is produced by our E. coli expression system and the target gene encoding Met130-Arg239 is expressed. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Mouse |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM Tris, 200mM NaCl, pH 8.0. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | NGF |
Entrez | 18049 |
UniProt ID | P01139 |
Protein Sequence | MGEFSVCDSV SVWVGDKTTA TDIKGKEVTV LAEVNINNSV FRQYFFETKC RASNPVESGC RGIDSKHWNS YCTTTHTFVK ALTTDEKQAA WRFIRIDTAC VCVLSRKATR |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM Tris, 200mM NaCl, pH 8.0. |
Alternative names | NGFB; Beta-nerve growth factor; Beta-NGF Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
41.8 kDa | |
E.coli |
Greater than 95% as determined by SDS-PAGE. | |
12.4 kDa | |
E.coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |