Mouse NGF protein

SKU: orb392186

Description

Recombinant mouse NGF protein is produced by our E. coli expression system and the target gene encoding Met130-Arg239 is expressed.

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: E. coli
• Target: NGF
• Reactivity: Mouse
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Protein Sequence: MGEFSVCDSV SVWVGDKTTA TDIKGKEVTV LAEVNINNSV FRQYFFETKC RASNPVESGC RGIDSKHWNS YCTTTHTFVK ALTTDEKQAA WRFIRIDTAC VCVLSRKATR

Storage & Handling

• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μM filtered solution of 20mM Tris, 200mM NaCl, pH 8.0.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NGFB; Beta-nerve growth factor; Beta-NGF

Quick Database Links

Gene Symbol

NGF

UniProt

P01139

NCBI Gene ID

18049