Mouse monoclonal antibody to HPV-18 E7

SKU: orb1108648

Description

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IF, IP, WB
Dilution Range	ELISA 0.05 to 0.1 µg/ml; WB 1 µg/ml; IF 0.33 to 1 µg/ml; IP 2 µg/ml. 10A8 gives a strong secondary signal in IF Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	10A8
Immunogen	Recombinant full-length HPV-18 E7-protein purified from E. coli
Purification	Protein G purification
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS pH 7.4, with 0.1% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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IF analysis of HPV-18 E7 protein in U2OS

IP analysis of HPV-18 E7 protein

Western-Blot analysis of HPV-18 E7 protein

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Immunoprecipitation

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