Med15 Antibody (C-term)

SKU: orb1926552

Description

Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:2000, IHC-P - 1:25
• Reactivity: Mouse, Rat
• Predicted Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD73393
• Immunogen: This Med15 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 712-746 amino acids from the C-terminal region of mouse Med15.
• Target: Med15
• Molecular Weight: 86607 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Mediator of RNA polymerase II transcription subunit 15, Mediator complex subunit 15, Positive cofactor 2 glutamine/Q-rich-associated protein, PC2 glutamine/Q-rich-associated protein, mPcqap, Med15, Pcqap

Anti-Med15 Antibody (C-term) at 1:1000 dilution + mouse testis lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 87 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Med15 Antibody (C-term) at 1:2000 dilution. Lane 1: mouse brain lysates. Lane 2: mouse spleen lysates. Lane 3: mouse thymus lysates. Lane 4: rat lung lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 87 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining (Mouse) Med15 in Mouse heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Quick Database Links

Gene Symbol

Med15

UniProt

Q924H2