Mouse Mapk11 Antibody (N-term)

• Catalog Number: orb1936293
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB34635
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Human
• Reactivity: Mouse
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 41397 Da
• Target: This Mouse Mapk11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of mouse Mapk11.
• UniProt ID: Q9WUI1
• NCBI: NP_035291.4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Mitogen-activated protein kinase 11, MAP kinase 11
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysate from mouse NIH/3T3 cell line, using Mouse Mapk11 Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.

Flow cytometric analysis of SH-SY5Y cells using Mouse Mapk11 Antibody (N-term) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Western blot analysis of lysates from mouse spleen, mouse heart tissue lysate (from left to right), using Mapk11 Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.

Mouse Mapk11 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse live tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of Mouse Mapk11 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.