Mouse Lats2 Antibody(C-term)

• Catalog Number: orb1932326
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB40566
• Tested applications: IHC-P, WB
• Reactivity: Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, IHC-P: 1:100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 115472 Da
• Target: This Mouse Lats2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1000-1027 amino acids from the C-terminal region of mouse Lats2.
• UniProt ID: Q7TSJ6
• NCBI: NP_056586.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Serine/threonine-protein kinase LATS2, Kinase phos
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis on paraffin-embedded Mouse testis tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-Mouse Lats2 Antibody (C-term) at 1:2000 dilution. Lane 1: Mouse ovary tissue lysate. Lane 2: Mouse testis tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 115 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.