Mouse Klf4 Antibody (Center)

SKU: orb1926632

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB - 1:1000, FC - 1:25
• Reactivity: Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD72168
• Immunogen: This Mouse Klf4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 321-354 amino acids from the Central region of mouse Klf4.
• Target: Klf4
• Molecular Weight: 51880 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor, Klf4, Ezf, Gklf, Zie

Western blot analysis of lysate from mouse heart tissue lysate, using Klf4 Antibody (Center). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.

Western blot analysis of lysates from mouse small intestine, mouse testis tissue lysate (from left to right), using Klf4 Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.

Overlay histogram showing NIH/3T3 cells stained (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Quick Database Links

Gene Symbol

Klf4

UniProt

Q60793