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Catalog Number | orb707127 |
---|---|
Category | Proteins |
Description | Recombinant Mouse CD25 Protein is produced by mammalian expression system and the target gene encoding Glu22-Lys236 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Mouse |
Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | IL2RA |
Entrez | 16184 |
UniProt ID | P01590 |
Protein Sequence | ELCLYDPPEV PNATFKALSY KNGTILNCEC KRGFRRLKEL VYMRCLGNSW SSNCQCTSNS HDKSRKQVTA QLEHQKEQQT TTDMQKPTQS MHQENLTGHC REPPPWKHED SKRIYHFVEG QSVHYECIPG YKALQRGPAI SICKMKCGKT GWTQPQLTCV DEREHHRFLA SEESQGSRNS SPESETSCPI TTTDFPQPTE TTAMTETFVL TMEYKHHHHH H |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Alternative names | Interleukin-2 receptor subunit alpha protein, IL-2 Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
FC, IHC, WB | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Unconjugated | |
90% | |
26.6 kDa | |
Mouse IL-2 R alpha, His Tag (orb570281) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Lys 236 (Accession # P01590-1). |
ELISA, ICC, IF, IHC | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 50.8 kDa after removal of the signal peptide. The apparent molecular mass of mIL2RA-hFc is approximately 70-100 kDa due to glycosylation. | |
Mammalian |
> 90% as determined by SDS-PAGE | |
25.8 kDa |