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Anti-Il2ra Antibody

Catalog Number: orb1147770

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb1147770
CategoryAntibodies
DescriptionAnti-Il2ra Antibody. Tested in ELISA, IF, IHC, ICC applications. This antibody reacts with Mouse, Rat.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, ICC, IF, IHC
ReactivityMouse, Rat
IsotypeRabbit IgG
ImmunogenE.coli-derived mouse Il2ra recombinant protein (Position: E22-I268).
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW50 kDa
UniProt IDP01590
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesGTP-binding nuclear protein Ran; Androgen receptor
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NoteFor research use only
Application notesImmunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Mouse ELISA, 0.1-0.5 μg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
Expiration Date12 months from date of receipt.
Anti-Il2ra Antibody

IF analysis of Il2ra using anti-Il2ra antibody. Il2ra was detected in an immunocytochemical section of RAW264.7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL rabbit anti-Il2ra Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-Il2ra Antibody

IHC analysis of Il2ra using anti-Il2ra antibody. Il2ra was detected in a paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Il2ra Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Anti-Il2ra Antibody

IHC analysis of Il2ra using anti-Il2ra antibody. Il2ra was detected in a paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Il2ra Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

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