Mouse IgM Isotype Control FITC Antibody

SKU: orb154495

Description

Mouse monoclonal antibody conjugated to FITC which recognizes IgM that is the major component of the natural antibodies and is also the first class of antibodies produced during a primary antibody response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen.

Images & Validation

• Tested Applications: FC
• Application Notes:
  Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.

Key Properties

• Antibody Type: Secondary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgM
• Clone No.: PFR-03
• Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Example of nonspecific mouse IgM (PFR-03) FITC signal on human peripheral blood; surface staining, 1 µg/ml.

Quick Database Links

NCBI Gene ID

35