Mouse IgG2b Isotype Control FITC Antibody

SKU: orb154492

Description

Mouse monoclonal antibody conjugated to FITC which recognizes IgG2b that belongs to many immunoglobulin G. It can be used as a negative control, to distinguish specific from non-specific binding of mouse IgG2b fluorochrome-conjugated antibodies to human cells. It was found that IgG2a was superior to IgG1 in activating complement. IgG2 is an antibody moleculer induced by infection.

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Tested Applications

Application Notes

Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.

Key Properties

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Antibody Type	Secondary Antibody
Clonality	Monoclonal
Isotype	Mouse IgG2b kappa
Clone No.	MPC-11
Immunogen	KLH-coupled trinitrophenol
Purification	Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Storage & Handling

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Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Buffer/Preservatives	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration	1 mg/ml
Disclaimer	For research use only

Mouse IgG2b isotype control Fluorescein Antibody [orb343782]

Applications:	FC
Conjugation:	FITC
Purity:	This product has been prepared from normal mouse serum by immunoaffinity chromatography using antibodies coupled to agarose beads. Extensive cross-adsorption was performed to remove any unwanted subclasses. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgM, and IgA. Fluorescein Conjugated Mouse IgG2b was proven by Dot Blot.
Source:	Mouse

Sizes Available: 100 μg

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Mouse IgG2b Isotype Control FITC Antibody

Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using mouse IgG2b Isotype control (MPC-11) FITC antibody (concentration in sample 8 μg/ml).

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Flow Cytometry

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