Mouse IgG2b isotype control Allophycocyanin Antibody

• Catalog Number: orb343802
• Category: Proteins
• Description: Mouse IgG2b isotype control Allophycocyanin Antibody
• Isotype: IgG2b
• Conjugation: APC
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgM, and IgA. Allophycoerythrin Conjugated Mouse IgG2b was proven by Dot Blot.
• Tested applications: DOT, ELISA, FC
• Dilution range: ELISA: 1:2000 - 1:20,000, FC: 1:1000-1:5000
• Application notes: Mouse IgG2b kappa isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Flow Cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
• Source: Mouse
• Biological Origin: Mouse
• Storage: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
• Alternative names: allophycocyanin conjugated Mouse Isotype control,
• Research Area: Protein Biochemistry
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Dot Blot Results of Mouse IgG2b Isotype Control Allophycocyanin Conjugated. Mouse IgG2b isotype APC Conj (1) 100 ng, (2) 33.33 ng, (3) 11.11 ng, (4) 3.70 ng, (5) 1.23 ng. Exposure: 1 second with DyLight 650 filter.