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Mouse IgG2b isotype control Allophycocyanin Antibody

Catalog Number: orb343802

DispatchUsually dispatched within 5-10 working days
$ 850.00
Catalog Numberorb343802
CategoryProteins
DescriptionMouse IgG2b isotype control Allophycocyanin Antibody
Tested applicationsDOT, ELISA, FC
IsotypeIgG2b
Concentration1.0 mg/mL
Dilution rangeELISA: 1:2000 - 1:20,000, FC: 1:1000-1:5000
Form/AppearanceLiquid (sterile filtered)
PurityMouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgM, and IgA. Allophycoerythrin Conjugated Mouse IgG2b was proven by Dot Blot.
ConjugationAPC
SourceMouse
Biological OriginMouse
StorageStore vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
Buffer/Preservatives0.01% (w/v) Sodium Azide
Alternative namesallophycocyanin conjugated Mouse Isotype control,
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NoteFor research use only
Application notesMouse IgG2b kappa isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Flow Cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Expiration Date12 months from date of receipt.
Mouse IgG2b isotype control Allophycocyanin Antibody

Dot Blot Results of Mouse IgG2b Isotype Control Allophycocyanin Conjugated. Mouse IgG2b isotype APC Conj (1) 100 ng, (2) 33.33 ng, (3) 11.11 ng, (4) 3.70 ng, (5) 1.23 ng. Exposure: 1 second with DyLight 650 filter.