Mouse IgG2b isotype control Allophycocyanin Antibody

SKU: orb343802

Description

Mouse IgG2b isotype control Allophycocyanin Antibody

Images & Validation

• Tested Applications: DOT, ELISA, FC
• Application Notes:
  Mouse IgG2b kappa isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Flow Cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Key Properties

• Source: Mouse
• Biological Origin: Mouse
• Isotype: IgG2b
• Purity: Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgM, and IgA. Allophycoerythrin Conjugated Mouse IgG2b was proven by Dot Blot.
• Conjugation: APC

Storage & Handling

• Storage: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Disclaimer: For research use only

Alternative Names

allophycocyanin conjugated Mouse Isotype control, APC control, mouse IgG2b subclass isotype control

Dot Blot Results of Mouse IgG2b Isotype Control Allophycocyanin Conjugated. Mouse IgG2b isotype APC Conj (1) 100 ng, (2) 33.33 ng, (3) 11.11 ng, (4) 3.70 ng, (5) 1.23 ng. Exposure: 1 second with DyLight 650 filter.