Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated

SKU: orb347574

Description

Mouse IgG1 antibody (Alkaline Phosphatase)

Images & Validation

• Tested Applications: DOT, ELISA, IHC, WB
• Dilution Range: ELISA: 1:5,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Reactivity: Mouse
• Application Notes:
  Anti-Mouse IgG1 Alkaline Phosphatase Antibody has been tested by ELISA, dot blot, and western blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Mouse IgG1 heavy chain
• Purity: IgG1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
• Conjugation: AP

Storage & Handling

• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Rabbit Anti-Mouse IgG1 (Gamma 1 chain) Antibody alkaline phosphatase Conjugation, Rabbit Anti-Mouse IgG1 alk phos Conjugated Antibody

Dot Blot of Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated. Antigen: Mouse IgG1. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.4 ng Lane 5 - 2.5 ng. Primary antibody: none. Secondary antibody: Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated at 1:1000 for one hour at RT. Block: orb348637 for 60 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.

Western Blot of Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated. Lane 1: Mouse IgG1. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated at 1:1000 o/n at 4°C. Block: orb348637 for 30 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds. Predicted/Observed size: 55 kDa for Mouse IgG1.