Mouse IgG1 Isotype Control APC Antibody

SKU: orb154438

Description

Mouse IgG1 Isotype control conjugated to APC which recognizes IgG1 that can be used for magnetic labeling and subsequent separation of cells, which are labeled with a primary mouse IgG1 antibody and also for positive selection or depletion of mouse B cells expressing IgG1 on the cell surface.

Images & Validation

• Tested Applications: FC
• Application Notes:
  Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.

Key Properties

• Antibody Type: Secondary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: MOPC-21
• Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: APC

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 0.1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using mouse IgG1 Isotype control (MOPC-21) APC antibody (concentration in sample 15 μg/ml).

Quick Database Links

NCBI Gene ID

35