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Catalog Number | orb22216 |
---|---|
Category | Proteins |
Description | Chromatographically purified Mouse IgG. Purified Mouse IgG may be used as a reference antigen, calibrator, coating protein and blocking agent in a variety of immunoassays including immunodiffusion, immunoelec- trophoresis, ELISA, Western blotting, dot-immunobinding assays (DIBA), haemagglutination, and cell-binding assays. |
Form/Appearance | Lyophilized, stable white powder. |
Hazard Information | This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Biorbyt accepts no liability for any inaccuracies or omissions in this information. |
RRID | AB_10748660 |
Storage | This product is shipped lyophilized at ambient temperature. After arrival store at +4°C. Prolonged storage may be at -20°C. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium. Reconstituted solution may be stored frozen. Working dilutions may be prepared by adding the required amount of phosphate buffered saline (PBS), should not be refrozen and preferably used within 24 hours. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. |
Buffer/Preservatives | Lyophilized, stable white powder. |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, IHC, IP, SDS-PAGE, WB | |
Unconjugated | |
Mouse IgG was prepared from normal mouse serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse Serum. | |
Mouse |
> 97% as determined by SDS-PAGE and HPLC. | |
13.5 kDa | |
E.Coli |
Greater than 95% as determined by SDS-PAGE. | |
13.4 kDa | |
E.coli |
Greater than 95% as determined by SDS-PAGE. | |
14.6 kDa | |
Mammalian cell |