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Catalog Number | orb347354 |
---|---|
Category | Antibodies |
Description | Mouse IgG + IgM (H&L) antibody (TRITC) |
Clonality | Polyclonal |
Species/Host | Goat |
Isotype | IgG |
Conjugation | TRITC |
Reactivity | Mouse |
Form/Appearance | Lyophilized |
Concentration | 1.0 mg/mL |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Purity | Anti-MOUSE IgG + IgM (H&L) (GOAT) Antibody was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse IgM. No reaction was observed against bovine, horse or human serum proteins. |
Immunogen | Mouse IgG and IgM whole molecules |
Tested applications | DOT, FC, FLISA, IF |
Dilution range | FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000 |
Application notes | Anti-MOUSE IgG + IgM (H&L) (GOAT) Rhodamine Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. A working dilution of 1:15,000 to 1:70,000 of the concentration is suggested for this product. Mouse IgG and IgM antibody is suitable for immunoassays including western blot and ELISA. |
Antibody Type | Secondary Antibody |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Alternative names | Goat Anti-Mouse IgG + IgM Antibody Fluorescein Con Read more... |
Note | For research use only |