Mouse IgG (H&L) Secondary Antibody

SKU: orb347515

Description

MOUSE IgG (H&L) antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:20,000 - 1:400,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Reactivity: Mouse
• Application Notes:
  Anti-Mouse IgG Antibody has been tested by ELISA and is ideal for Western Blotting, Immunohistochemistry, ELISA as well as other antibody detection methods.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Mouse IgG whole molecule
• Purity: Anti-Mouse IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 2.08 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Mouse IgG Antibody, rabbit anti-mouse IgG heavy and light chain antibody, rb anti-ms IgG heavy and light chain, Anti-Mouse Secondary Antibody, rabbit anti Mouse IgG Antibody

ELISA results of purified Rabbit anti-Mouse IgG Antibody tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG (p/n orb2652749). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n orb347654) and TMB substrate (p/n orb348651).

FcγRIV-dependent uptake and activation of kidney, but not dermal, macrophages by small immune complexes, ‘auto' antibodies, and NZWxBXSB serum. (H) TNF staining in kidney F4/80bright macrophages from wt mice 1 hour after i.v. injection of the indicated amounts of BSA, RaBSA, BSA;RaBSA, rabbit IgG (Ra-IgG), rabbit anti-mouse transferrin (Ra-mTransferrin) [p/n orb345374], rabbit anti-mouse albumin (Ra-mAlbumin) [p/n orb2652784], rabbit anti-mouse IgG (Ra-mIgG) [p/n orb347515] or Ra- mIgG (Fab')2 [p/n orb346783]. N = 4–5 mice per treatment.