Mouse IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated

SKU: orb347435

Description

MOUSE IgG (H&L) antibody (Alkaline Phosphatase)

Images & Validation

• Tested Applications: DOT, ELISA, IHC, WB
• Dilution range: ELISA: 1:15,000, IHC: 1:200 - 1:1,000, WB: 1:1,000 - 1:4,000
• Reactivity: Mouse
• Application Notes:
  Mouse secondary antibody conjugated to Alkaline Phosphatase is available in a variety of formats. Anti-Mouse IgG Alkaline Phosphatase Antibody has been tested by ELISA, dot blot, and western blot and is suitable for ELISA, Immunohistochemistry, Western Blotting as well as other Alkaline Phosphatase antibody based assays.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Mouse IgG whole molecule
• Purity: Anti-Mouse Conjugated Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Mouse IgG and Mouse Serum.
• Conjugation: AP

Storage & Handling

• Storage: Store secondary antibody conjugate at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Disclaimer: For research use only

Alternative Names

Goat Anti-Mouse IgG Antibody alkaline phosphatase Conjugation, Goat Anti-Mouse IgG alk phos Conjugated Antibody

Western Blot of Alkaline Phosphatase Conjugated Goat Anti-Mouse IgG Antibody Lane 1: Mouse IgG Load: 100 ng per lane Secondary antibody: Alkaline Phosphatase Conjugated Goat Anti-Mouse IgG Antibody at 1:1000 for 60 min at RT Block: orb348637 for 30 min RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa