Mouse IgG F(c) antibody

Catalog Number: orb347516

Catalog Number	orb347516
Category	Antibodies
Description	Mouse IgG F(c) antibody
Species/Host	Rabbit
Clonality	Polyclonal
Tested applications	ELISA, IHC, WB
Reactivity	Mouse
Isotype	IgG
Immunogen	Anti-Mouse IgG F(c) was produced by repeated immunization with mouse IgG F(c) fragment in rabbit.
Concentration	2.1 mg/mL
Dilution range	ELISA: 1:100,000 - 1:250,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Form/Appearance	Liquid (sterile filtered)
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab).
Conjugation	Unconjugated
Storage	Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Alternative names	rabbit anti-Mouse IgG F(c) Antibody, Rb-a-Mouse F( Read more...
Note	For research use only
Application notes	Mouse IgG F(c) Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.
Expiration Date	12 months from date of receipt.

Applications:	ELISA, IHC, SDS-PAGE, WB
Conjugation:	Unconjugated
Purity:	MOUSE IgG F(c) fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, anti-Mouse IgG and anti-Mouse IgG F(c). No reaction was observed against anti-Mouse IgG F(ab’)2 or anti-Papain.
Source:	Mouse

Sizes Available: 1 mg

Conjugation:	FITC
Purity:	This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Mouse IgG, anti-Mouse IgG F(c) and anti-Mouse Serum. No reaction was observed against anti-Mouse IgG F(ab’)2 or anti-Papain.
Source:	Mouse

Sizes Available: 1 mg

Conjugation:	HRP
Purity:	This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Mouse IgG, anti-Mouse IgG F(c) and anti-Mouse Serum. No reaction was observed against anti-Mouse IgG F(ab’)2 or anti-Papain.
Source:	Mouse

Sizes Available: 1 mg

Applications:	DOT
Conjugation:	Biotin
Purity:	This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Mouse IgG, anti-Mouse IgG F(c) and anti-Mouse Serum. No reaction was observed against anti-Mouse IgG F(ab’)2 or anti-Papain.
Source:	Mouse

Sizes Available: 1 mg