Mouse IgG Fc Antibody

• Catalog Number: orb347516
• Category: Antibodies
• Description: Mouse IgG F(c) antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 2.1 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab).
• Immunogen: Anti-Mouse IgG F(c) was produced by repeated immunization with mouse IgG F(c) fragment in rabbit.
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:100,000 - 1:250,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application notes: Mouse IgG F(c) Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Alternative names: rabbit anti-Mouse IgG F(c) Antibody, Rb-a-Mouse F(
• Note: For research use only

ELISA of Rabbit Anti-Mouse IgG F(c) Antibody tested against purified Mouse IgG F(c). Each well was coated in duplicate with 1.0 µg of Mouse IgG F(c) (p/n orb346280) [Red Line], Mouse IgG Fab (p/n orb346282) [Green line], Mouse IgG F(ab')2 (p/n orb346281) [Blue Line], and Mouse IgG (p/n orb2652749) [Purple Line]. The working dilution of Mouse IgG F(c) is 1:53000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Rabbit IgG HRP conjugated (p/n orb347654), and TMB substrate (p/n orb348651).