Mouse IgG F(ab')2 Antibody Fluorescein Conjugated

SKU: orb347424

Description

Mouse IgG F(ab')2 antibody (FITC)

Images & Validation

• Tested Applications: FC, FLISA, IF
• Dilution Range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Reactivity: Mouse
• Application Notes:
  This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Mouse IgG F(ab')2 fragment
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG, Mouse IgG F(ab’)2 and Mouse Serum. No reaction was observed against Mouse IgG F(c).
• Conjugation: FITC

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 2.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Goat Anti-Mouse IgG F(ab')2 fluorescein Conjugated Antibody, Goat Anti-Mouse IgG Fab2 fluorescein Conjugated Antibody, Goat Anti-Mouse IgG Fab2 Fragment Antibody FITC Conjugation

Immunofluorescence (IF) micrographs of skeletal fibers labeled with different antibodies to I-band titin (T12, N2A, and PEVK, red IF staining), and Z-disk marker α-actinin (ACTN2, green IF staining). Shown are examples of Wt, Het, and Hom fibers after TEV-protease treatment, held passively for 30 min at a stretched length. Scale bars, 5 µm.

Mutant titin expression in skeletal muscles of different genotypes. (B) Correlative immunofluorescence (IF) and immunogold electron microscopy of wild-type (Wt), heterozygous (Het), and homozygous (Hom) psoas muscle. Representative IF images of fibers (colored panels) labeled with HaloTag antibody (green) and counterstained for α-actinin (ACTN2, red), and immunoelectron micrograph showing HaloTag labeling. Scale bars, 5 µm (IF); 1 µm (IEM).