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Catalog Number | orb735042 |
---|---|
Category | Proteins |
Description | Mouse IFN-alpha 1, His Tag is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Lys 189 (Accession # P01572-1). |
Reactivity | Mouse |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 21.0 kDa |
Target | IFN-alpha 1 |
UniProt ID | P01572 |
Source | Mouse IFN-alpha 1, His Tag (orb735042) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Lys 189 (Accession # P01572-1). |
Expression System | HEK293 |
Biological Origin | Mouse |
Biological Activity | Loaded Mouse IFN-alpha 1, His Tag on HIS1K Biosensor, can bind Human IFNAR1, Fc Tag with an affinity constant of 0.17 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
Expression Region | Cys 24 - Lys 189 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_034632.2 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | IFN-alpha 1,IFNA1,IFNalpha 1,IFN-alpha-1,LeIF D Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21.0 kDa. The protein migrates as 24-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Loaded Mouse IFN-alpha 1, His Tag (Cat. No. orb735042) on HIS1K Biosensor, can bind Human IFNAR1, Fc Tag (Cat. No. orb257558) with an affinity constant of 0.17 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Unconjugated | |
95% | |
26.6 kDa | |
Mouse IFN-alpha / beta R2, His Tag (orb1087551) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Ala 242 (Accession # O35664-1). |
Unconjugated | |
90% | |
47.6 kDa | |
Mouse IFN-alpha / beta R1, His Tag (orb1087552) is expressed from human 293 cells (HEK293). It contains AA Glu 27 - Thr 429 (Accession # P33896-1). |