Mouse Hmga2 Antibody (N-term)

• Catalog Number: orb1926742
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB51072
• Tested applications: WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:2000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 11819 Da
• Target: This Mouse Hmga2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 26-59 amino acids from the N-terminal region of Mouse Hmga2.
• UniProt ID: P52927
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: High mobility group protein HMGI-C, High mobility
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysate from NCCIT cell line, using Mouse Hmga2 Antibody (N-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.

Western blot analysis of lysates from HepG2, mouse NIH/3T3 cell line (from left to right), using Mouse Hmga2 Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.

All lanes: Anti-Hmga2 Antibody (N-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysates. Lane 2: 293T whole cell lysates. Lane 3: MDA-MB-231 whole cell lysates. Lane 4: NIH/3T3 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 12 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.