Mouse Hmga2 Antibody (C-term)

• Catalog Number: orb1788118
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: 72-105 aa
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:25, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 11819 Da
• Target: This Mouse Hmga2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 72-105 amino acids from the C-terminal region of Mouse Hmga2.
• UniProt ID: P52927
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: High mobility group protein HMGI-C, High mobility
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Fluorescent image of C2C12cells stained with Mouse Hmga2 Antibody (C-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

All lanes: Anti-Hmga2 Antibody (C-term) at 1:1000 dilution. Lane 1: F9 whole cell lysates. Lane 2: HepG2 whole cell lysates. Lane 3: NCCIT whole cell lysates. Lane 4: NIH/3T3 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 12 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.