You have no items in your shopping cart.
Cart summary
Item 1 of 3
Item 1 of 3
Mouse Fap Antibody(N-term)
Catalog Number: orb1932162
| Catalog Number | orb1932162 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB40867 |
| Tested applications | IHC-P, WB |
| Reactivity | Mouse |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:2000, WB: 1:500, IHC-P: 1:100 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 87945 Da |
| Target | This Mouse Fap antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-41 amino acids from the N-terminal region of mouse Fap. |
| UniProt ID | P97321 |
| NCBI | NP_032012.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Prolyl endopeptidase FAP, Fap {ECO:0000312|MGI:MGI Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis on paraffin-embedded Mouse kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Anti-Mouse Fap Antibody (N-term) at 1:2000 dilution + mouse kidney lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 88 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-Mouse Fap Antibody (N-term) at 1:500 dilution. Lane 1: Mouse kidney lysate. Lane 2: Mouse ovary lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 88 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
